Protein concentration after FPLC. This dramatically increased our protein concentration so that enzyme assay can be carried out
Results of the protein characterization are shown below (from a long time ago)
In lane 8 it looks like we have a dimer but it did not affect our FPLC. FtHap is approximately 45 kDa which is where one of our lines is shown in lane 8.
Lane 1: n/a
Lane 2: ladder (smo671)
Lane 3: sample 1
Lane 4: n/a
Lane 5: sample 3
Lane 6: sample 4
Lane 7: n/a
Lane 8: elution 1
Lane 9: elution 2
Lane 10: n/a
Week 12:
This Week I worked on FPLC and concentrated my protein here are the results form FPLC
FPLC results for FtHAP: The tubes from 30 to 38 were pulled because they contained protein
FPLC nanodrop result before concentrating sample
Week 11:
Divya - show the alignment as a screen shot to preserve the formatting. An alignement doesn't mean much when it is out of alignment. Dr. B 11/19/12
I worked on my homology model and prepared it for virtual screening. Additionally I characterized FTHAP and concentrated the protein to be used for FPLC
The results from the Homology model are shown below:
Score E Sequences producing significant alignments: (bits) Value
ExPDB|1auiA|2.1| HUMAN CALCINEURIN HETERODIMER 336 2e-92 ExPDB|1m63A|2.8| Crystal structure of calcineurin cyclophilin cy... 335 2e-92 ExPDB|1tcoA|2.5| TERNARY COMPLEX OF A CALCINEURIN A FRAGMENT CA... 335 4e-92 ExPDB|1wao1|2.9| PP5 STRUCTURE 193 2e-49 ExPDB|1s95B|1.6| Structure of serine threonine protein phosphata... 191 8e-49 ExPDB|3v4yG|2.098| Crystal Structure of the first Nuclear PP1 ho... 188 6e-48 ExPDB|1fjmB|2.1| Protein serine threonine phosphatase 1 alpha i... 187 8e-48 ExPDB|3p71C|2.7| Crystal structure of the complex of LCMT 1 and ... 187 1e-47 ExPDB|3v4yC|2.098| Crystal Structure of the first Nuclear PP1 ho... 187 1e-47 ExPDB|3c5wC|2.8| Complex between PP2A specific methylesterase PM... 186 2e-47 ExPDB|2o8gA|2.5| Rat pp1c gamma complexed with mouse inhibitor 2 186 3e-47 ExPDB|2nppF|3.3| Structure of the Protein Phosphatase 2A Holoenzyme 185 4e-47 ExPDB|1u32A|2| Crystal structure of a Protein Phosphatase 1 Cal... 184 1e-46 ExPDB|3fgaC|2.7| Structural Basis of PP2A and Sgo interaction 184 1e-46 ExPDB|2iaeC|3.5| Crystal structure of a protein phosphatase 2A ... 183 2e-46 ExPDB|1s70A|2.7| Complex between protein ser thr phosphatase 1 ... 180 1e-45 ExPDB|3icfA|2.3| Structure of Protein serine threonine phosphata... 159 3e-39
>[Template]|1auiA|2.1| HUMAN CALCINEURIN HETERODIMER Length = 378 [Display Alignment in DeepView] Score = 336 bits (861), Expect = 2e-92, Method: Composition-based stats. Identities = 165/372 (44%), Positives = 234/372 (62%), Gaps = 26/372 (6%) Query: 17 PPPLWCEAKREALIDGKGKVNLEVMLVHFLRQGRLSKHDALNIIQNASSVLRTEPNVLRI 76 PP AK + D GK ++++ H +++GRL + AL II +S+LR E N+L ISbjct: 11 PPSHRLTAKE--VFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDI 68 Query: 77 ADPSVVVVGDVRGQFYDLAKIIFIGNMFSRAKT-YLFLGNYIDSCFFSTECILLLLAAKL 135 D V V GD+ GQF+DL K+ +G S A T YLFLG+Y+D +FS EC+L L A K+Sbjct: 69 -DAPVTVCGDIHGQFFDLMKLFEVGG--SPANTRYLFLGDYVDRGYFSIECVLYLWALKI 125 Query: 136 THPSCVFLLRGNHECRFMSNIFDFRGECLKKYNDDVYEAIMTAFDCLPLAAVVNNQYFCV 195 +P +FLLRGNHECR ++ F F+ EC KY++ VY+A M AFDCLPLAA++N Q+ CVSbjct: 126 LYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCV 185 Query: 196 HGGLSPDVTSVDNIRLIYRFREPPSKGAMCDLLWSDPFWDVENPSSVCEGSRDDYYTPGN 255 HGGLSP++ ++D+IR + RF+EPP+ G MCD+LWSDP D N Sbjct: 186 HGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEK--------------- 230 Query: 256 GPSYGTAPCFLDNEQRGCSYLFNYHSVKHFLLTNGLLCVVRSHEVQDDGYKLYRFNSVSN 315 T F N RGCSY ++Y +V FL N LL ++R+HE QD GY++YR + + Sbjct: 231 -----TQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTG 285 Query: 316 FPCMMSVFSAPNYCDNLHNKGAVLILEGKQIGIKQFCCSPHPYVLPKHLNAFEWSFSYLL 375 FP ++++FSAPNY D +NK AVL E + I+QF CSPHPY LP ++ F WS ++ Sbjct: 286 FPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVG 345 Query: 376 DSVRDIFASIIS 387 + V ++ ++++Sbjct: 346 EKVTEMLVNVLN 357
Better alignment:
Query: 17 PPPLWCEAKREALIDGKGKVNLEVMLVHFLRQGRLSKHDALNIIQNASSVLRTEPNVLRI 76
PP AK + D GK ++++ H +++GRL + AL II +S+LR E N+L I
Sbjct: 11 PPSHRLTAKE--VFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDI 68
Query: 77 ADPSVVVVGDVRGQFYDLAKIIFIGNMFSRAKT-YLFLGNYIDSCFFSTECILLLLAAKL 135
D V V GD+ GQF+DL K+ +G S A T YLFLG+Y+D +FS EC+L L A K+
Sbjct: 69 -DAPVTVCGDIHGQFFDLMKLFEVGG--SPANTRYLFLGDYVDRGYFSIECVLYLWALKI 125
Query: 136 THPSCVFLLRGNHECRFMSNIFDFRGECLKKYNDDVYEAIMTAFDCLPLAAVVNNQYFCV 195
+P +FLLRGNHECR ++ F F+ EC KY++ VY+A M AFDCLPLAA++N Q+ CV
Sbjct: 126 LYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCV 185
Query: 196 HGGLSPDVTSVDNIRLIYRFREPPSKGAMCDLLWSDPFWDVENPSSVCEGSRDDYYTPGN 255
HGGLSP++ ++D+IR + RF+EPP+ G MCD+LWSDP D N
Sbjct: 186 HGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEK--------------- 230
Query: 256 GPSYGTAPCFLDNEQRGCSYLFNYHSVKHFLLTNGLLCVVRSHEVQDDGYKLYRFNSVSN 315
T F N RGCSY ++Y +V FL N LL ++R+HE QD GY++YR + +
Sbjct: 231 -----TQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTG 285
Query: 316 FPCMMSVFSAPNYCDNLHNKGAVLILEGKQIGIKQFCCSPHPYVLPKHLNAFEWSFSYLL 375
FP ++++FSAPNY D +NK AVL E + I+QF CSPHPY LP ++ F WS ++
Sbjct: 286 FPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVG 345
Query: 376 DSVRDIFASIIS 387
+ V ++ ++++
Sbjct: 346 EKVTEMLVNVLN 357</span>
Week 10:
Since the PCR failed yet again, A surrogate protein was used form now on. FTHAP was experssed and purified this week. Next week, FPLC will be run. I also started my homology model. Week 9:
This week I redid my primary and secondary PCR at both 56 and 57 degrees. Results to be posted soon. Week 8:
102112 - Divya, I would also do another primary at 57 degrees - then do a couple of different secondary's (from your original primary and from this new primary). -- DR. B
I Gel checked my primary and secondary PCR and got the following results
Lane 1: Skip
Lane 2: 100 bp ladder
Lane 3: Primary PCR
Land 4:Secondary PCR
Results: The secondary PCR did not show on the gel, so I think I need to change the annealing temperature. I will have 2 samples one will be 56 degrees with from the old and new PCR and I will do the same for 57 degrees. Week 7:
101612 - Divya, show some of your results/gels. -- Dr. B
This week I diluted my primers, made an oligomix, and ran my primary PCR. I will gel check it with secondary PCR. The results from both primary and secondary PCR will be posted next week. Week 6:
100912 - Divya - ok, not a great gel - but move on to cloning PCRs so taht you can catch up with the others. - Dr. B
This week I did my second PCR practice this week and next week I will dilute my primers and begin PCR^2 on my target (Finally). I need to make an oligomix as well.
The results of my second PCR are shown below. there is a small crack at the bottom but it does not affect the results obtained.
Skip
DNA Ladder 100kp
Sample A Lowest concentration
Sample B Medium concentration
Sample C Highest concentration
Sample D control No DNA
Week 5:
093012- Divya. Good but show some results here. Dr. B
This week I did a PyMol refresher and I completed the primer design for pNIC-Bsa4 cloning. The results were posted to google docs. I had a lot of tests this week so I could not get too much done. Next week I will do PCR2
Divya - in midi-prep you are purifying plasmid DNA instead of protein. Also, your PCR is ~ok. I don't think you have contamination but rather some smearing of the DNA. The sharp bands are actually about the right size (the GBR22 gene is about 1,000 bp). So - good job. You can move on to PCR#2. Also, eventually you might need to re-do the Midiprep if you need more for your cloning. -- Dr. B - good labeling of your marker too next to the PCR gel. Could specify exactly what it is you are amplifying in the PCR caption.
This week I did midi-prep and I did my first PCR practice. The protein concentration from midi-prep yielded low protein concentration, which was most likely due to the fact that too much elution buffer was added initially.
The results of the midi-prep are shown above. I obtained a relatively protein yeild of 37.1 ng/ul
The 2 images above are from the results of the PCR. It looks like there may be some contamination.
Skip
Ladder 100bp
Sample A
Sample B
Sample C
Sample D (No DNA)
Week 3:
Divya - ok - good image. Include brief analysis of image. Are the bands correct? Any anomalies? Also, you get some back credit for last week. -- Dr. B 091812
This week I Did transformation of E-coli and I completed the RE Digest. I was not successful in transforming my bacteria so I borrowed bacteria form another person in lab (initials SB). I then I grew the bacteria in LB Broth and spun down my sample to obtain a pellet, which I then froze in -20 C fridge. In the RE Digest I ran a gel electrophoresis which is pictured below: The 100bp ladder is shown on the left. gel Results for pGBR22 DNA (above) (Fixed the lane numbers) Lane 1; Skip Lane 2; 100 bp NEB DNA Ladder Lane 3; Plasmid Lane 4; 25ul control Lane 5; 25ul EcoRI Lane 6; 25ul PvuII Lane 7; 25ul EcoRI and PvuII
Week 2: I diluted Primer for pNIC-Bsa4 and prepared it for sequencing. I also analyzed the DNA sequence for PGBR-22. Week 1: I created my target page as shown below
Week 13:
This week I did concentration after FPLC
Results of the protein characterization are shown below (from a long time ago)
Lane 1: n/a
Lane 2: ladder (smo671)
Lane 3: sample 1
Lane 4: n/a
Lane 5: sample 3
Lane 6: sample 4
Lane 7: n/a
Lane 8: elution 1
Lane 9: elution 2
Lane 10: n/a
Week 12:
This Week I worked on FPLC and concentrated my protein here are the results form FPLC
Week 11:
Divya - show the alignment as a screen shot to preserve the formatting. An alignement doesn't mean much when it is out of alignment. Dr. B 11/19/12
I worked on my homology model and prepared it for virtual screening. Additionally I characterized FTHAP and concentrated the protein to be used for FPLC
The results from the Homology model are shown below:
Score E
Sequences producing significant alignments: (bits) Value
ExPDB|1auiA|2.1| HUMAN CALCINEURIN HETERODIMER 336 2e-92
ExPDB|1m63A|2.8| Crystal structure of calcineurin cyclophilin cy... 335 2e-92
ExPDB|1tcoA|2.5| TERNARY COMPLEX OF A CALCINEURIN A FRAGMENT CA... 335 4e-92
ExPDB|1wao1|2.9| PP5 STRUCTURE 193 2e-49
ExPDB|1s95B|1.6| Structure of serine threonine protein phosphata... 191 8e-49
ExPDB|3v4yG|2.098| Crystal Structure of the first Nuclear PP1 ho... 188 6e-48
ExPDB|1fjmB|2.1| Protein serine threonine phosphatase 1 alpha i... 187 8e-48
ExPDB|3p71C|2.7| Crystal structure of the complex of LCMT 1 and ... 187 1e-47
ExPDB|3v4yC|2.098| Crystal Structure of the first Nuclear PP1 ho... 187 1e-47
ExPDB|3c5wC|2.8| Complex between PP2A specific methylesterase PM... 186 2e-47
ExPDB|2o8gA|2.5| Rat pp1c gamma complexed with mouse inhibitor 2 186 3e-47
ExPDB|2nppF|3.3| Structure of the Protein Phosphatase 2A Holoenzyme 185 4e-47
ExPDB|1u32A|2| Crystal structure of a Protein Phosphatase 1 Cal... 184 1e-46
ExPDB|3fgaC|2.7| Structural Basis of PP2A and Sgo interaction 184 1e-46
ExPDB|2iaeC|3.5| Crystal structure of a protein phosphatase 2A ... 183 2e-46
ExPDB|1s70A|2.7| Complex between protein ser thr phosphatase 1 ... 180 1e-45
ExPDB|3icfA|2.3| Structure of Protein serine threonine phosphata... 159 3e-39
>[Template]|1auiA|2.1| HUMAN CALCINEURIN HETERODIMER Length = 378 [Display Alignment in DeepView] Score = 336 bits (861), Expect = 2e-92, Method: Composition-based stats. Identities = 165/372 (44%), Positives = 234/372 (62%), Gaps = 26/372 (6%) Query: 17 PPPLWCEAKREALIDGKGKVNLEVMLVHFLRQGRLSKHDALNIIQNASSVLRTEPNVLRI 76 PP AK + D GK ++++ H +++GRL + AL II +S+LR E N+L ISbjct: 11 PPSHRLTAKE--VFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDI 68 Query: 77 ADPSVVVVGDVRGQFYDLAKIIFIGNMFSRAKT-YLFLGNYIDSCFFSTECILLLLAAKL 135 D V V GD+ GQF+DL K+ +G S A T YLFLG+Y+D +FS EC+L L A K+Sbjct: 69 -DAPVTVCGDIHGQFFDLMKLFEVGG--SPANTRYLFLGDYVDRGYFSIECVLYLWALKI 125 Query: 136 THPSCVFLLRGNHECRFMSNIFDFRGECLKKYNDDVYEAIMTAFDCLPLAAVVNNQYFCV 195 +P +FLLRGNHECR ++ F F+ EC KY++ VY+A M AFDCLPLAA++N Q+ CVSbjct: 126 LYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCV 185 Query: 196 HGGLSPDVTSVDNIRLIYRFREPPSKGAMCDLLWSDPFWDVENPSSVCEGSRDDYYTPGN 255 HGGLSP++ ++D+IR + RF+EPP+ G MCD+LWSDP D N Sbjct: 186 HGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEK--------------- 230 Query: 256 GPSYGTAPCFLDNEQRGCSYLFNYHSVKHFLLTNGLLCVVRSHEVQDDGYKLYRFNSVSN 315 T F N RGCSY ++Y +V FL N LL ++R+HE QD GY++YR + + Sbjct: 231 -----TQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTG 285 Query: 316 FPCMMSVFSAPNYCDNLHNKGAVLILEGKQIGIKQFCCSPHPYVLPKHLNAFEWSFSYLL 375 FP ++++FSAPNY D +NK AVL E + I+QF CSPHPY LP ++ F WS ++ Sbjct: 286 FPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVG 345 Query: 376 DSVRDIFASIIS 387 + V ++ ++++Sbjct: 346 EKVTEMLVNVLN 357
Better alignment:
Query: 17 PPPLWCEAKREALIDGKGKVNLEVMLVHFLRQGRLSKHDALNIIQNASSVLRTEPNVLRI 76 PP AK + D GK ++++ H +++GRL + AL II +S+LR E N+L I Sbjct: 11 PPSHRLTAKE--VFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDI 68 Query: 77 ADPSVVVVGDVRGQFYDLAKIIFIGNMFSRAKT-YLFLGNYIDSCFFSTECILLLLAAKL 135 D V V GD+ GQF+DL K+ +G S A T YLFLG+Y+D +FS EC+L L A K+ Sbjct: 69 -DAPVTVCGDIHGQFFDLMKLFEVGG--SPANTRYLFLGDYVDRGYFSIECVLYLWALKI 125 Query: 136 THPSCVFLLRGNHECRFMSNIFDFRGECLKKYNDDVYEAIMTAFDCLPLAAVVNNQYFCV 195 +P +FLLRGNHECR ++ F F+ EC KY++ VY+A M AFDCLPLAA++N Q+ CV Sbjct: 126 LYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCV 185 Query: 196 HGGLSPDVTSVDNIRLIYRFREPPSKGAMCDLLWSDPFWDVENPSSVCEGSRDDYYTPGN 255 HGGLSP++ ++D+IR + RF+EPP+ G MCD+LWSDP D N Sbjct: 186 HGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEK--------------- 230 Query: 256 GPSYGTAPCFLDNEQRGCSYLFNYHSVKHFLLTNGLLCVVRSHEVQDDGYKLYRFNSVSN 315 T F N RGCSY ++Y +V FL N LL ++R+HE QD GY++YR + + Sbjct: 231 -----TQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTG 285 Query: 316 FPCMMSVFSAPNYCDNLHNKGAVLILEGKQIGIKQFCCSPHPYVLPKHLNAFEWSFSYLL 375 FP ++++FSAPNY D +NK AVL E + I+QF CSPHPY LP ++ F WS ++ Sbjct: 286 FPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVG 345 Query: 376 DSVRDIFASIIS 387 + V ++ ++++ Sbjct: 346 EKVTEMLVNVLN 357</span>Week 10:
Since the PCR failed yet again, A surrogate protein was used form now on. FTHAP was experssed and purified this week. Next week, FPLC will be run. I also started my homology model.
Week 9:
This week I redid my primary and secondary PCR at both 56 and 57 degrees. Results to be posted soon.
Week 8:
102112 - Divya, I would also do another primary at 57 degrees - then do a couple of different secondary's (from your original primary and from this new primary). -- DR. B
I Gel checked my primary and secondary PCR and got the following results
Lane 1: Skip
Lane 2: 100 bp ladder
Lane 3: Primary PCR
Land 4:Secondary PCR
Results: The secondary PCR did not show on the gel, so I think I need to change the annealing temperature. I will have 2 samples one will be 56 degrees with from the old and new PCR and I will do the same for 57 degrees.
Week 7:
101612 - Divya, show some of your results/gels. -- Dr. B
This week I diluted my primers, made an oligomix, and ran my primary PCR. I will gel check it with secondary PCR. The results from both primary and secondary PCR will be posted next week.
Week 6:
100912 - Divya - ok, not a great gel - but move on to cloning PCRs so taht you can catch up with the others. - Dr. B
This week I did my second PCR practice this week and next week I will dilute my primers and begin PCR^2 on my target (Finally). I need to make an oligomix as well.
The results of my second PCR are shown below. there is a small crack at the bottom but it does not affect the results obtained.
Week 5:
093012- Divya. Good but show some results here. Dr. B
This week I did a PyMol refresher and I completed the primer design for pNIC-Bsa4 cloning. The results were posted to google docs. I had a lot of tests this week so I could not get too much done. Next week I will do PCR2
Results from the Pymol Refresher are shown here:
Week 4:
Divya - in midi-prep you are purifying plasmid DNA instead of protein. Also, your PCR is ~ok. I don't think you have contamination but rather some smearing of the DNA. The sharp bands are actually about the right size (the GBR22 gene is about 1,000 bp). So - good job. You can move on to PCR#2. Also, eventually you might need to re-do the Midiprep if you need more for your cloning. -- Dr. B - good labeling of your marker too next to the PCR gel. Could specify exactly what it is you are amplifying in the PCR caption.
This week I did midi-prep and I did my first PCR practice. The protein concentration from midi-prep yielded low protein concentration, which was most likely due to the fact that too much elution buffer was added initially.
Week 3:
Divya - ok - good image. Include brief analysis of image. Are the bands correct? Any anomalies? Also, you get some back credit for last week. -- Dr. B 091812
This week I Did transformation of E-coli and I completed the RE Digest. I was not successful in transforming my bacteria so I borrowed bacteria form another person in lab (initials SB). I then I grew the bacteria in LB Broth and spun down my sample to obtain a pellet, which I then froze in -20 C fridge. In the RE Digest I ran a gel electrophoresis which is pictured below:
gel Results for pGBR22 DNA (above) (Fixed the lane numbers)
Lane 1; Skip
Lane 2; 100 bp NEB DNA Ladder
Lane 3; Plasmid
Lane 4; 25ul control
Lane 5; 25ul EcoRI
Lane 6; 25ul PvuII
Lane 7; 25ul EcoRI and PvuII
Week 2: I diluted Primer for pNIC-Bsa4 and prepared it for sequencing. I also analyzed the DNA sequence for PGBR-22.
Week 1: I created my target page as shown below
Disease: Trypansoma brucei
Enzyme: Protein Phosphatase 2B
Essentiality: Essential, used for cytokinesis
Not complex
EC#: 3.1.3.16
Drugability: 0.8 (yes)
Assay:
http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Enzyme_Assay/calcineurin.Par.0001.File.tmp/calcineurin.pdf
http://www.brenda-enzymes.org/php/result_flat.php4?ecno=3.1.3.16
crystal structure:
Amino Acid sequence:
MLPILKAGEDEGGPRIPPPLWCEAKREALIDGKGKVNLEVMLVHFLRQGRLSKHDALNIIQNASSVLRTE PNVLRIADPSVVVVGDVRGQFYDLAKIIFIGNMFSRAKTYLFLGNYIDSCFFSTECILLLLAAKLTHPSC VFLLRGNHECRFMSNIFDFRGECLKKYNDDVYEAIMTAFDCLPLAAVVNNQYFCVHGGLSPDVTSVDNIR LIYRFREPPSKGAMCDLLWSDPFWDVENPSSVCEGSRDDYYTPGNGPSYGTAPCFLDNEQRGCSYLFNYH SVKHFLLTNGLLCVVRSHEVQDDGYKLYRFNSVSNFPCMMSVFSAPNYCDNLHNKGAVLILEGKQIGIKQ FCCSPHPYVLPKHLNAFEWSFSYLLDSVRDIFASIISCEGA
Gene Sequence:
ATGCTGCCTATACTAAAGGCCGGGGAAGACGAGGGGGGTCCCCGGATCCCCCCTCCTCTATGGTGTGAGG CGAAGCGTGAGGCGTTAATCGATGGCAAAGGAAAAGTTAATCTTGAGGTAATGCTTGTTCATTTTCTTCG GCAAGGACGTTTGAGCAAGCACGATGCACTCAACATAATTCAAAATGCATCAAGTGTACTTCGTACGGAA CCCAACGTCCTGAGGATTGCTGATCCTTCGGTTGTAGTCGTCGGCGACGTTCGGGGACAGTTCTACGATT TAGCAAAAATTATATTTATAGGTAATATGTTCAGTAGGGCAAAAACGTATTTGTTTCTAGGTAATTATAT TGACTCATGTTTTTTTAGCACCGAGTGTATACTCCTTTTGCTTGCGGCGAAACTAACCCACCCGAGTTGT GTATTTTTGCTTCGTGGAAACCATGAATGCCGTTTTATGTCGAATATTTTCGATTTTCGGGGAGAGTGTC TGAAGAAATATAATGACGATGTTTACGAAGCCATTATGACAGCGTTTGATTGTCTTCCACTGGCAGCTGT TGTAAACAATCAGTATTTTTGTGTACATGGAGGTCTTTCACCTGACGTTACGAGTGTTGACAATATTCGC TTAATTTATCGATTTCGGGAACCCCCGTCGAAAGGGGCTATGTGTGATTTATTATGGTCTGATCCGTTTT GGGATGTAGAGAATCCGTCGTCAGTTTGCGAAGGTTCTAGAGATGATTATTACACCCCTGGAAATGGCCC ATCGTACGGTACGGCGCCATGCTTTCTTGACAACGAGCAGAGAGGTTGCAGTTATTTGTTTAACTATCAT AGTGTAAAGCACTTTTTGTTGACGAATGGTCTTCTTTGCGTCGTACGTAGCCATGAGGTTCAAGATGATG GTTATAAGTTGTATAGGTTCAACAGTGTTTCCAATTTTCCGTGCATGATGTCCGTTTTTTCTGCCCCTAA TTACTGTGATAACTTGCACAACAAGGGTGCAGTCCTCATTCTTGAGGGGAAACAAATAGGTATAAAGCAG TTTTGTTGCTCGCCTCATCCTTATGTTCTCCCAAAGCATCTTAACGCATTTGAATGGTCATTCTCTTACC TGTTGGATAGTGTTCGCGACATATTTGCTTCCATCATTTCGTGTGAGGGAGCATGA